Ngari virus (Orthobunyavirus, Peribunyaviridae) in ixodid ticks collected from cattle in Guinea

Ngari virus is a mosquito-borne belonging to the genus Orthobunyavirus (Peribunyaviridae family). This pathogenic humans and causes severe illness. present in several African countries, including Madagascar. Here, we report detection of ixodid ticks collected from cows Guinea. A tick survey was conducted March-November 2018 six regions The sample comprised 710 pools, with total 2067 five species 197 cows. At initial stage, screened subsample pools vector-borne viruses multiplex genus-specific primer panel. In second stage study, narrowed search all samples by qPCR for virus. All positive were sequenced primers flanking virus-specific fragments on S M segments. We found 12 that formed engorged livestock three villages Kindia Kankan regions. Sequencing segments confirmed detected belong virus, most similar strain Adrar, which isolated Mauritania. viral RNA following species: Amblyomma variegatum, Rhipicephalus geigyi, Rh. (Boophilus) spp. There no evidence are competent vectors Most likely, obtained through blood an infected host. study can be recommended as simpler approach wide screening subsequent testing cattle mosquitos those locations where PCR-positive collected.